Laser Scanning Confocal Microscope Magnification

Laser scanning confocal microscopy laser scanning confocal microscopes employ a pair of pinhole apertures to limit the specimen focal plane to a confined volume approximately a micron in size.

Laser scanning confocal microscope magnification.

This means that we can view visual sections of tiny structures that. Capturing multiple two dimensional images at different depths in a sample enables the. Clsm combines high resolution optical imaging with depth selectivity which allows us to do optical sectioning. In the past the traditional laser microscope excited the whole thickness of the sample resulting in saturated blurry images and sometimes visualizing false colocalization images.

Relatively thick specimens can be imaged in successive volumes by acquiring a series of sections along the optical z axis of the microscope. The confocal laser scanning microscope clsm is a microscope which focuses only on a single focal plane and the unfocused plane will not be visualized. The laser scans across the object and an image is built up pixel by pixel on a screen. Confocal microscopy most frequently confocal laser scanning microscopy clsm or laser confocal scanning microscopy lcsm is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out of focus light in image formation.

Fluorescent microscopy not only makes our images look good it also allows us to gain a better understanding of cells structures and tissue.