Laser Scanning Microscope Magnification And Resolution

The confocal laser scanning microscope.

Laser scanning microscope magnification and resolution.

With confocal laser scanning microscopy clsm we can find out even more. In the confocal laser scanning microscope the highest frequency to be sampled f is imposed by the optical system and for a particular resolution specification. This first generation instrument images corneal structures at 400 magnification and has a field of view of 400 400 µm when used with a 63 objective lens that has a numerical aperture of 0 9. Laser scanning confocal microscopy laser scanning confocal microscopes employ a pair of pinhole apertures to limit the specimen focal plane to a confined volume approximately a micron in size.

This means that we can view visual sections of tiny structures that. Fluorescent microscopy not only makes our images look good it also allows us to gain a better understanding of cells structures and tissue. This technique captures the surface image and enables magnification control by reducing the scan area to smaller sizes thus increasing magnification without any resolution loss. Clsm combines high resolution optical imaging with depth selectivity which allows us to do optical sectioning.

Relatively thick specimens can be imaged in successive volumes by acquiring a series of sections along the optical z axis of the microscope. In some cases specimens should be sampled at more than 2 3 times the highest information frequency to allow for the possibility that the highest frequency was misjudged. It uses a 670 nm red wavelength helium neon. The laser scanning microscope uses a scanning design called beam scanning where the laser image path is scanned in a raster pattern on the surface of the sample.