Laser Scanning Microscope Maximum Magnification

Laser scanning confocal microscopy laser scanning confocal microscopes employ a pair of pinhole apertures to limit the specimen focal plane to a confined volume approximately a micron in size.

Laser scanning microscope maximum magnification.

A confocal laser scanning microscope or also known as laser scanning confocal microscope is used to obtain high resolution images and generate 3d reconstructions through direct optical sectioning to provide clear images from a range of depths of thicker specimens for nanometer level imaging and measurement. It has a maximum magnification of about 100000. Fluorescent microscopy not only makes our images look good it also allows us to gain a better understanding of cells structures and tissue. A scanning electron microscope sem produces a 3d image of a sample by bouncing electons off and dectecting them at multiple detectors.

Confocal laser scanning fluorescence microscope. Radiation in a lm. This means that we can view visual sections of tiny structures that. The confocal laser scanning microscope clsm is a microscope which focuses only on a single focal plane and the unfocused plane will not be visualized.

Lscm laser scanning confocal microscope parameter value comment maximum ip pixel resolution 1270 x 1000 pixel user adjustable maximum field of view 1 ccd equivalent 9 6 x 12 8mm. The maximum magnification of a light microscope. Lets you look at thin slices in a sample while keeping sample intact. This feature is termed the zoom factor and is usually employed to adjust the image spatial resolution by altering the scanning laser sampling period.

With confocal laser scanning microscopy clsm we can find out even more. In the past the traditional laser microscope excited the whole thickness of the sample resulting in saturated blurry images and sometimes visualizing false colocalization images. Relatively thick specimens can be imaged in successive volumes by acquiring a series of sections along the optical z axis of the microscope. Lets you look specifically at parts of a cell such as individual proteins by labelling them with fluorescence.

Light microscope laser scanning confocal microscope transmission electron microscope tem and scanning electron microscope sem light. A transmission electron microscope tem produces a 2d image of a thin sample and has a maximum resolution of 500000. Lens used in a lm. The maximum magnification of a tem.

Clsm combines high resolution optical imaging with depth selectivity which allows us to do optical sectioning.